Intestinal trefoil factor confers colonic epitheliai resistance to apoptosis

摘要

Intestinal trefoil factor (lTF) is an essential regulator of colonic epithelial restitution, the rapid migration of colonocytes over mucosal wounds. High levels of ITF are frequently present in colorectal cancers and derived cell lines. Mucosal restitution re- quires the detachment of epithelium from substrate. which would be expected to induce apoptosis. However, mice deficient in lTF showed an increase in colonocyte apoptosis unaccompanied by changes in expression of receptor-related (TNFR/Fas) or stress- related (Bcl-family) cell death regulators. An ITF-expressing colonic (HT-lTF1) cell line was resistant to apoptosis induced by serum starvation and ceramide. Exogenous ITF also protected another human colonic carcinoma-derived cell line (HCT116) and a non- transformed rat intestinal epithelial cell line (IEC-6) from apoptosis.