Targeting and imaging single biomolecules in living cells by complementation-activated light microscopy with split-fluorescent proteins

摘要

Light microscopy offers sensi tive and noninvasive means to probe molecular events in cells. With the rapid emergence of single-molecule (SM) fluores cence and nanoscopy techni ques, the field of cellular biology is undergoing a revolution in imaging. In cells, imaging in dividual biomolecules permits their localization with an accu racy of a few nanometers and provides outstanding molecular details on the organization and dynamics of nanoscale biolog ical processes, normally not accessible by conventional dif fraction-limited optical imaging.