The single Cys_2-His_2 zinc finger domain of the GAGA protein flanked by basic residues is sufficient for high-affinity specific DNA binding

摘要

Specific DNA binding to the core consensus site GAGAGAG has been shown with an 82-residue peptide (residues 310-391) taken from the Drosophila transcription factor GAGA. Using a series of deletion mutants, it was demonstrated that the minimal domain required for specific binding (residues 310-372) includes a single zinc finger of the Cys_2-His_2 family and a stretch of basic amino acids located on the N-terminal end of the zinc finger.