Selection and nuclear immobilization of exportable RNAs

摘要

The intracellular distribution of RNAs de- pends on interactions of cis-acting nuclear export elements or nuclear retention elements with trans-acting nuclear trans- port or retention factors. To learn about the relationship between export and retention, we isolated RNAs that are exported from nuclei of Xenopus laevis oocytes even when most RNA export is blocked by an inhibitor of Ran-dependent nucleocytoplasmic transport, the Matrix protein of vesicular stomatitis virus. Export of the selected RNAs is saturable and specific.