Rer1p as common machinery for the endoplasmic reticulum localization of membrane proteins

摘要

Rer1p, a Golgi membrane protein, is re- quired for the correct localization of an endoplasmic reticu- lum (ER) membrane protein, Sec12p, by a retrieval mecha- nism from the cis-Golgi to the ER. To test whether or not the role of Rer1p is common to multiple ER membrane proteins, we examined the localization of two other ER membrane proteins, Sec71p and Sec63p, in the wild-type and rer1 mutant yeast cells, using their fusions with an -mating factor pre- cursor (Mf1p). Although Sec71p and Sec63p have completely different topology from Sec12p, their Mf1p fusion proteins were also mislocalized to the trans-Golgi in the rer1 mutant.