Direct and GTP-dependent interaction of ADP ribosylation factor 1 with coatomer subunit β

摘要

A site-directed photocrosslink approach was used to elucidate components that interact directly with ADP- ribosylation factor (ARF)-GTP during coat assembly. Two ARF mutants were generated that contain a photolabile amino acid at positions distant to each other within the ARF molecule. Here we show that one of the two positions specif- ically interacts with coatomer subunit β both on Golgi mem- branes and in isolated coat protein complex type I (COPI)- coated vesicles.