Manganese Peroxidase Production by Immobilized Phanerochaete chrysosporium BKM-F-1767 in a Cell Bioreactor

摘要

Manganese-dependent peroxidase (MnP) production was performed in an immobilized cell bioreactor in which Phanerochaete chrysosporium BKM-F-1767 was immobilized on polystyrene foam. The immobilized cell culture yielded significantly greater MnP activity than the conventional stationary liquid culture. Cultivation was carried out in batch mode; the effect of glucose concentration was investigated and growth kinetics parameters were found as, μ_max=0.59 day-1, K_s=0.33 g/L and K_ss=14.5. Batch operation led to maximum MnP (770.82 U/L) in the culture medium containing 0.05% Tween 80, 10 g/L glucose, and 174 µM Mn2+ at 37°C and pH 4.5. Enzyme productivity was obtained as 110.12 U/day/L.