HISTOPATHOLOGICAL FEATURE OF GILLS OF AFRICAN CATFISH (CLARIAS GARIEPINUS) EXPOSED TO LEAD NITRATE

摘要

This study aims to determine changes in gill damage of African catfish (Clarias gariepinus) exposed to lead nitrate.This study used 24 African catfish with an average weight of 20-25 g,size 10-12 cm,age ± two months.This study uses a completely randomized design (CRD).All African catfish populations were divided into four groups,each consisting of six replications,namely P1,P2,P3,and P0 as negative controls,each P1 was given a lead nitrate concentration of 7.26 mg/L,P2 was given 14.53 mg/L,and P3 given 29.06 mg/L.Based on macroscopic observations,it shows that the gills become swollen and pale.Histopathological picture of the gills was examined under a light microscope at a magnification of 100 times.The scoring method uses the Semiquantitative Histopathological Scoring Method to check for edema,hyperplasia and lamella fusion.Then,the Crucial Wallis test was carried out followed by the Mann Whitney test.Analysis of the average edema P0(0.30),P1(1.90),P2(1.60),and P3(0.50).The average number of hyperplasia P0(0.30),P1 (2.30),P2(2.20) and P3(1.00).The average number of lamella fusion P0(0.10),P1(0.70),P2(1.60) and P3(3.40).From these results it can be concluded that the most severe lamella edema damage occurs at a concentration of 7.26 mg/L,and the worst lamella hyperplasia occurs at a concentration of 7.26 mg/L and 14.53 mg/L,and the worst lamella fusion occurs at a concentration of 29.06 mg/L.