The Evaluation Of Methods For Detectionof Bacillus Anthracis Spores In Artificiallyrncontaminated Soil Samples

摘要

The nested PCR has been used to evaluate the usefulness and efficiency of different Bacillus anthracis spore isolation methods in contaminated soil samples.The best results were obtained using two methods described by Beyer et al.[1] and Cheun et al.[9].Outer and inner pairs of primers were designed from the protective antigen gene of plasmid pXO1 as well as from genes B and C of the capsule region of the plasmid pXO2.The influence of soil types on obtained results was also studied.The type of soil samples did not affect the nested PCR results.Furthermore,the sensitivity of nested PCR and PCR-ELISA was also examined.