Toxicological Affects of Essential Oils from Eucalyptus Eucalyptus globules and Clove Eugenia caryophyllus on Albino Rats

摘要

The present work was planned to determine the median lethal doses (LD_(50)) and to evaluate the haema-tological and biochemical changes, and histopathological effects of the essential oils from eucalyptus Eucalyptus globules L. and clove Eugenia caryophyllus on liver and kidney of albino rats. The LD_(50) was 2,334.4 and 3,597.5 mg/kg b.w. of eucalyptus and clove oils, respectively. Obtained data revealed that 1/10 LD_(50) of both tested oils resulted in a significant increase in WBC counts and produced a significant decrease in haemoglobin concentration and platelets count at 5th and 10th doses, as well as RBC counts (-17.1 and -9.4% below normal level) at 10th dose. The activities of serum GOT and GPT enzymes were a significant increase at 5th and 10th doses in treated rats by both tested oils. While two essential oils had mild effect on kidney function, these oils produced a significant increase in creatinine and urea concentration at the 10th dose. Histopathological studies on liver and kidney revealed that both essential oils caused relatively moderate pathological changes in the liver as congestion of the blood vessels in the portal area associated with inflammatory infiltration. Also, two tested oils induced desquamation of the epithelial cells of the renal tubules.