ACCUMULATION OF IXERIN F AND ACTIVITIES OF SOME TERPENOKD BIOSYNTHETIC ENZYMES IN A CELL SUSPENSION CULTURE OF LACTUCA VIROSA L.

摘要

A cell suspension culture of Lactuca virosa L. (Asteraceae, tribe Lactuceae) is capable of synthesizing sesquiterpene lac- tones of which ixerin F is the main compound. The culture was characterized on growth (by dissimilation rates), on ixerin F accumulation (by RP-HPLC) and on some enzyme activities involved in early steps of terpenoid biosynthesis. Acetoace- Byl-coenzyme A thiolase (AACT, E. C. 2.3.1.9) and 3S-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGS,E.C. 4.1.3.5) activities of the cells were assayed spectrophotometrically.