n nnnReorganization of the actin and microtubule networks is knownto occur in targeted vascular parenchymal root cells upon infectionwith the nematode Meloidogyne incognita. Here, we show thatactin-depolymerizing factor (ADF) is upregulated in the giantfeeding cells of Arabidopsis thaliana that develop upon nematodeinfection and that knockdown of a specific ADF isotype inhibitsnematode proliferation. Analysis of the levels of transcriptand the localization of seven ADF genes shows that five areupregulated in galls that result from the infection and thatADF2 expression is particularly increased between 14 and 21d after nematode inoculation. Further analysis of ADF2 functionin inducible RNA interference lines designed to knock down ADF2expression reveals that this protein is required for normalcell growth and plant development. The net effect of decreasedlevels of ADF2 is F-actin stabilization in cells, resultingfrom decreased F-actin turnover. In nematode-infected plantswith reduced levels of ADF2, the galls containing the giantfeeding cells and growing nematodes do not develop due to thearrest in growth of the giant multinucleate feeding cells, whichin turn is due to an aberrant actin network.展开▼
机译:ABSTRACTn FONT> TH> TR> TABLE> n
n TOP n <字体颜色= 464c53>抽象 FONT> n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n FONT> TH> TR> TABLE> n nnn肌动蛋白和微管网络的重组已知 SUP>线虫根结线虫感染后 SUP>感染了目标血管实质根细胞。在这里,我们显示 SUP>肌动蛋白解聚因子(ADF)在由线虫发育的拟南芥 I>的巨型 SUP>饲养细胞中被上调。 SUP>感染,而特定ADF同种型的敲除抑制了 SUP>线虫的增殖。分析转录本 SUP>的水平以及七个 ADF I>基因的定位,结果表明,有五个是 SUP>在受感染的胆囊中被上调,而 > SUP> ADF2 I>的表达在接种线虫后14到21 SUP> d之间特别增加。进一步设计用于敲低 ADF2 I> SUP>表达的诱导性RNA干扰品系中ADF2功能 SUP>的进一步分析显示,该蛋白是正常所需的 SUP>细胞生长和植物发育。降低ADF2的 SUP>水平的净作用是细胞中F-肌动蛋白的稳定,这是由于F-肌动蛋白周转减少导致的 SUP>。在ADF2含量降低的线虫感染植物 SUP>中,由于 SUP>的捕食,没有形成包含巨型 SUP>饲养细胞和正在生长的线虫的the虫。 SUP>的巨大多核饲养细胞的生长又是由于肌动蛋白网络异常引起的。 SUP>
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Unité Mixte de Recherches Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université de Nice-Sophia Antipolis, F-06903 Sophia Antipolis, France;
Laboratory of Plant Cell Biology, Wageningen University, 6703 BD Wageningen, The Netherlands|School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United Kingdom;
Unité Mixte de Recherches Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université de Nice-Sophia Antipolis, F-06903 Sophia Antipolis, France;
Unité Mixte de Recherches Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université de Nice-Sophia Antipolis, F-06903 Sophia Antipolis, France;
School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United Kingdom;
Unité Mixte de Recherches Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université de Nice-Sophia Antipolis, F-06903 Sophia Antipolis, France;
Unité Mixte de Recherches Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université de Nice-Sophia Antipolis, F-06903 Sophia Antipolis, France;
School of Biological and Biomedical Sciences, University of Durham, Durham DH1 3LE, United Kingdom;
Unité Mixte de Recherches Interactions Biotiques et Santé Végétale, Institut National de la Recherche Agronomique, Centre National de la Recherche Scientifique, Université de Nice-Sophia Antipolis, F-06903 Sophia Antipolis, France;