n nnnMale germline development in angiosperms produces the pair ofsperm cells required for double fertilization. A key regulatorof this process in Arabidopsis thaliana is the male germline-specifictranscription factor DUO POLLEN1 (DUO1) that coordinates germcell division and gamete specification. Here, we uncover therole of DUO3, a nuclear protein that has a distinct, but overlappingrole with DUO1 in male germline development. DUO3 is a conservedprotein in land plants and is related to GON-4, a cell lineageregulator of gonadogenesis in Caenorhabditis elegans. Mutantduo3-1 germ cells either fail to divide or show a delay in division,and we show that, unlike DUO1, DUO3 promotes entry into mitosisindependent of the G2/M regulator CYCB1;1. We also show thatDUO3 is required for the expression of a subset of germlinegenes under DUO1 control and that like DUO1, DUO3 is essentialfor sperm cell specification and fertilization. Furthermore,we demonstrate an essential sporophytic role for DUO3 in celldivision and embryo patterning. Our findings demonstrate essentialdevelopmental roles for DUO3 in cell cycle progression and cellspecification in both gametophytic and sporophytic tissues.展开▼
机译：ABSTRACTn n
n TOP n <字体颜色= 464c53>抽象 n 介绍 n 结果 n 讨论 n 方法 n 参考文献 n n nnn被子植物中的雄性种系发育产生一对 精子双受精所需的细胞。拟南芥（Arabidopsis thaliana）中此过程的关键调节物 是雄性生殖系特异性 转录因子DUO POLLEN1（DUO1），可协调细菌 < / SUP>细胞分裂和配子规格。在这里，我们揭示了DUO3的 作用，该核蛋白在雄性种系发育中与DUO1具有独特但重叠的 作用。 DUO3是陆地植物中一种保守的 蛋白，与GON-4有关，GON-4是秀丽隐杆线虫中性腺生成的细胞谱系 调节剂。 突变的 duo3-1 生殖细胞未能分裂或显示出延迟，我们证明，与DUO1不同，DUO3促进进入有丝分裂 独立于G2 / M调节剂CYCB1; 1。我们还表明，在DUO1控制下表达 基因的子集需要 DUO3，与DUO1一样，DUO3对于精子也是必不可少的 细胞规格和受精。此外， 我们证明了DUO3在细胞 分裂和胚胎形成中的基本孢子体作用。我们的研究结果证明了DUO3在配子体和孢子体组织中细胞周期进程和细胞 规范中必不可少的 发育作用。展开▼
