Potential Errors in Electron Transport Rates Calculated from Chlorophyll Fluorescence as Revealed by a Multilayer Leaf Model

摘要

Increasingly, photosynthetic electron transport rate is being calculated from chlorophyll fluorescence measure-ments. The fluorescence signal is a complex mixture of contributions from different depths within the mesophyll. One condition required for electron transport calculated from fluorescence to represent the rate accurately is that the ratio of photosynthetic capacity to light absorbed be constant throughout the leaf. In order to explore the fluorescence properties of leaves where this assumption is not true, a new approximation for φPSII is used to generate F_m′ and F_s values throughout the leaf. F_s is assumed to be proportional to the amount of light absorbed from the fluorescence measuring beam and constant, i.e. indep-endent of the actinic irradiance or CO₂ concentration. This assumption is validated by measurements from Eucalyptus maculata, Flaveria bidentis and Triticum aestivum, with two different types of fluorometer, where irradiance or CO₂ response curves were measured with normal or inverted leaf orientations. The new approach enables fluorescence values to be generated at each layer in a multilayer model. Two applications using this approach are presented. First, the model is used to show that when quantum yield varies through a leaf, then fluorescence will lead to an incorrect estimate of electron transport rate. Secondly, since chlorophyll fluorescence is also used to calculate the CO₂ concentration at the sites of carboxyla-tion within chloroplasts, C_c, the model is also used to show that C_c may vary with depth. Significant variation in C_c through the mesophyll could lead to an apparent dependence of internal conductance on irradiance or CO₂.