A multi-array competitive immunoassay for the detection of broad-range molecular size organic compounds relevant for astrobiology

摘要

We have developed antibodies and a multi-array competitive immunoassay (MACIA) for the detection of a wide range of molecular size compounds, from single aromatic ring derivatives or polycyclic aromatic hydrocarbons (PAHs), through small peptides, proteins or whole cells (spores). Multiple microarrays containing target molecules are used simultaneously to run several competitive immunoassays. The sensitivity of the MACIA for small organic compounds like naphthalene, 4-phenilphenol or 4-tertbutilphenol is in the range of 100-500ppb (ng ml~(-1)), for others like the insecticide terbutryn it is at the ppt (ng l~(-1)) level, while for small peptides, as well as for more complex molecules like the protein thioredoxin, the sensitivity is approximately 1-2 ppb, or 10~4-10~5 spores of Bacillus subtilis per milliliter. For organic compounds, a water-methanol solution was used in order to achieve a better dissolution of the organics without compromising the antibody-antigen interaction. The above-mentioned compounds were detected by MACIA in water-(10%) methanol extracts from spiked pyrite and hematite-containing rock powder samples, as well as from a spiked-sand sample subjected to organic extraction with dichloromethane-methanol (1/1).