Improved Nucleic Acid Triggered Probe Activation through the Use of a 5-Thiomethyluracil Peptide Nucleic Acid Building Block

摘要

To improve the efficiency of a nucleic acid triggered probe activation(NATPA)system a 5-thiomethyluracil peptide nucleic acid(PNA)building block has been synthesized.Attachment of imidazole and a coumarin ester to uracils at the ends of two PNAs resulted in a 550 000-fold acceleration of DNA-triggered coumarin release relative to imidazole and a 6-fold increase in k_cat relative to a system which had these groups attached to the amino and carboxy ends of PNAs.