A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm

摘要

A new carbazole-derived dicationic compound, namely 2,7-bis(l-hydroxyethyl-4-vinylpyridinium iodine)-iV-ethyl-carbazole (2,7-9£-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.