Site-selected incorporation of 5-carboxymethyl-aminomethyl(-2-thio)uridine into RNA sequences by phosphoramidite chemistryf

摘要

5-Carboxymethytaminomethyluridine (cmnm~5U) and 5-carboxy-methylaminomethyl-2-thiouridine (cmnm~5s~2U) are located at the wobble position in several cytosolic and mitochondrial tRNA sequences. In this paper, we report the first site-selected incorporation of cmnm~5U and cmnm~5s~2U into RNA sequences by phosphoramidite chemistry on a CPG solid support. Trifluoroacetyl and 2-(trimethylsilyl)ethyl were selected for the protection of the amine and carboxyI functions, respectively.