Imaging cortical electrical stimulation in vivo: fast intrinsic optical signal versus voltage-sensitive dyes

摘要

We applied high-temporal-resolution optical imaging utilizing both the fast intrinsic optical signal (fIOS)nand voltage-sensitive dyes (VSDs) to observe the spatiotemporal characteristics of rat somatosensory cortexnduring electrical stimulation. We find that changes in both the fIOS and VSD signals occur rapidly u0001u000130msu0002nafter the stimulus is applied, suggesting that both membrane depolarization and transmembrane ion move-nment occur shortly after the stimulus, preceding the more gradual physiological changes in oxygen consump-ntion revealed by the slower component of the intrinsic optical signal. We find that the VSD signal spreadsnthrough a much larger area of cortex than the fIOS. © 2008 Optical Society of America