Spectroscopic analysis of breath ethylene and oxidative stress relation with glycaemic status in type 2 diabetes

摘要

The paper presents the CO_2 laser absorption spectroscopy (CO_2LPAS) techniques as an effective tool for sensitive analysis of ethylene in human breath. Breath analysis is non-invasive method to monitor the disease states and evolution using biomarkers. Breath ethylene as a breath biomarker of oxidative stress was determined in the exhaled breath of subjects with type-2 diabetes and a healthy control group using a CO_2LPAS system. Characterization of the CO_2LPAS, and the selection of the ethylene absorption lines are described. All subjects involved in this study were subjected to breath analysis for determining the concentration of ethylene and blood tests to determine glucose levels and glycated hemoglobin Alc (HbAlc). Breath ethylene was found in high concentration in diabetics compared to healthy subjects and higher in subjects with type-2 diabetes with health complications. The data obtained by the CO_2LPAS system suggest the involvement of oxidative stress in the complications of diabetes, revealing that the increased breath ethylene has a close relationship with high glucose levels, as observed by the HbAlc levels. The CO_2LPAS system present a high sensitivity and high selectivity with fast measurement and can be used to monitor the molecular changes in diabetic subjects by measuring breath ethylene concentrations.