Isolation and Characterization of a-Amylase Producing Bacillus subtilis

摘要

Deptt. of Microbiology, Shri D. M. Burungale College of Science and Arts, Shegaon-444 203, Distt. Buldana, Maharashtra;%The present study is concerned with production of a-amylase by strains of Bacillus subtilis S5 (3). The fermentation was carried out by continuous shaking containing 25mL of medium in 250 mL Erlenmeyer flask. The maximum production of the enzyme was optimized at pH 7.5, while incubation temperature investigated was 42℃. The production of enzyme was obtained maximum at 48 hrs after incubation.