Ligninolytic Enzymes Production and Decolorization Potential of Native Fungi Isolated from Pulp and Paper Mill Sludge

摘要

In this study, potential of 30 fungal strains isolated from pulp and paper mill sludge were assessed as biolignolytic microorganisms and their ability to produce ligninolytic enzymes viz., laccase (Lcc), lignin peroxidase (LiP), manganese peroxidase (MnP), xylanase and cellulase using different chromogenic substrates. A total of eight fungi exhibited conspicuous halos with their respective substrate. The fungal isolates were found to be significantly different in qualitative enzyme production. Maximum enzyme activity for Lcc, MnP and LiP were 26.24, 8.5 and 12.99 U/mL exhibited by fungal isolates Trametes sp. (LDFD5), Nigrospora sp. (LDF00204) and Alternaria sp (LDFD4) on 10th and 13th day, respectively, by solid state fermentation (SSF). Conspicuous reduction in colour unit upto 75.29 % and 76.37 % were observed by fungal strain LDF00204 at 30℃ temperature and pH 5 respectively. Results depicted that fungal isolate Nigrospora sp. LDF00204 (Accession No. KP732542) exhibited a relatively noble characteristic of having ligninolytic enzymes, is able to degrade lignin and its co-compounds efficiently, and thus it could be utilized for decolorization of pulp and paper mill effluent and also for bioremediation of alike pollutants.