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首页> 外文期刊>Mycopathologia >An Improved Protocol for the Preparation of Total Genomic DNA from Isolates of Yeast and Mould Using Whatman FTA Filter Papers
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An Improved Protocol for the Preparation of Total Genomic DNA from Isolates of Yeast and Mould Using Whatman FTA Filter Papers

机译:使用Whatman FTA滤纸从酵母和霉菌分离物中制备总基因组DNA的改进方案

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摘要

Here, we present a significantly improved version of our previously published method for the extraction of fungal genomic DNA from pure cultures using Whatman FTA® filter paper matrix technology. This modified protocol is extremely rapid, significantly more cost effective than our original method, and importantly, substantially reduces the problem of potential cross-contamination between sequential filters when employing FTA technology.
机译:在这里,我们介绍了使用Whatman FTA ®滤纸基质技术从纯培养物中提取真菌基因组DNA的方法的显着改进。修改后的协议非常快速,比我们的原始方法具有更高的成本效益,并且重要的是,在采用FTA技术时,大大减少了顺序过滤器之间潜在的交叉污染问题。

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