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Functional Cooperativity of Enzymes of Phosphoinositide Conversion According to Synergistic Effects on Pectin Secretion in Tobacco Pollen Tubes

机译:根据对烟草花粉管中果胶分泌的协同效应,磷酸化磷酸酶转化酶的功能协同作用

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摘要

The Arabidopsis phosphoinositide kinases PI4Kβ1 and PIP5K5 have been implicated in the control of directional vesicle trafficking underlying polar tip growth in pollen tubes. PI4Kβ1 and PIP5K5 catalyze key consecutive steps of phosphoinositide conversion, and it appears obvious that phosphatidylinositol-4-phosphate formed by PI4Kβ1 might act as a substrate for phosphatidylinositol-4,5-bisphosphate formation by PIP5K5. However, this hypothesis has not been experimentally addressed and distinct localization patterns of PI4Kβ1, PIP5K5, and also Pl-synthases (PIS) generating phos-phatidylinositol suggest additional complexity. Here, the synergistic functionality of enzymes of phosphoinositide conversion was assessed. In tobacco and Arabidopsis pollen tubes, phosphoinositides influence the apical secretion of pectin, and increased pectin deposition results in characteristic morphological alterations. Catalytically active and dominant negative variants of PI4Kβ1 and PIP5K5 were systematically co-expressed in tobacco pollen tubes and the incidence of morphologies related to enhanced pectin secretion was evaluated. The data support a proposed functional interplay of PI4Kβ1 and PIP5K5 at the trans-Golgi network, mediating directional vesicle trafficking. Co-expression experiments additionally including PIS isoforms, PIS1 or PIS2, indicate that pectin secretion is synergistically mediated by PI4Kpβ1 and PIP5K5 acting on Ptdlns formed by PIS2 rather than PIS1. Possible ramifications for the preferential channeling of phosphoinositide intermediates between particular isoforms of PI pathway enzymes are discussed.
机译:拟南芥磷酸肌醇激酶PI4Kβ1和PIP5K5与花粉管中极尖生长的定向囊泡运输有关。 PI4Kβ1和PIP5K5催化磷酸肌醇转化的关键连续步骤,并且很明显,PI4Kβ1形成的磷脂酰肌醇4-磷酸可能充当PIP5K5形成磷脂酰肌醇-4,5-双磷酸的底物。然而,该假设尚未通过实验得到解决,PI4Kβ1,PIP5K5以及产生磷酸-phadydylinositol的PI合成酶(PIS)的独特定位模式表明了额外的复杂性。在这里,评估了磷酸肌醇转化酶的协同功能。在烟草和拟南芥花粉管中,磷酸肌醇会影响果胶的根尖分泌,果胶沉积增加会导致特征性的形态学改变。 PI4Kβ1和PIP5K5的催化活性和显性负变异在烟草花粉管中系统共表达,并评估了与果胶分泌增强有关的形态发生率。数据支持反式高尔基体网络中PI4Kβ1和PIP5K5的拟议功能相互作用,介导定向囊泡运输。共表达实验还包括PIS同工型PIS1或PIS2,表明果胶分泌由PI4Kpβ1和PIP5K5协同介导,作用于PIS2而非PIS1形成的Ptdlns。讨论了PI途径酶特定同工型之间磷酸肌醇中间体优先通道的可能后果。

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  • 来源
    《Molecular Plant》 |2010年第5期|p.870-881|共12页
  • 作者单位

    Department of Plant Biochemistry, Albrecht-von-Haller-lnstitute for Plant Sciences, Georg-August-University Gottingen, Justus-von-Liebig-Weg 11, 37077 Gottingen, Germany;

    Department of Plant Biochemistry, Albrecht-von-Haller-lnstitute for Plant Sciences, Georg-August-University Gottingen, Justus-von-Liebig-Weg 11, 37077 Gottingen, Germany,Present address: Center for Biochemistry, Cologne University, Joseph-Stelzmann-Strape 52, 50931 Cologne, Germany;

    Department of Plant Biochemistry, Albrecht-von-Haller-lnstitute for Plant Sciences, Georg-August-University Gottingen, Justus-von-Liebig-Weg 11, 37077 Gottingen, Germany,Present address: Department of Forest Botany, Georg-August-University Gottingen, Busgenweg 2, 37077 Gottingen, Germany;

    Department of Plant Biochemistry, Albrecht-von-Haller-lnstitute for Plant Sciences, Georg-August-University Gottingen, Justus-von-Liebig-Weg 11, 37077 Gottingen, Germany;

    Department of Plant Biochemistry, Albrecht-von-Haller-lnstitute for Plant Sciences, Georg-August-University Gottingen, Justus-von-Liebig-Weg 11, 37077 Gottingen, Germany,Present address: Department of Plant Cell Biology, Albrecht-von-Haller-lnstitute for Plant Sciences, Georg-August-University Gottingen, Untere Karspule 2,37073 Gottingen, Germany;

    Department of Plant Biochemistry, Albrecht-von-Haller-lnstitute for Plant Sciences, Georg-August-University Gottingen, Justus-von-Liebig-Weg 11, 37077 Gottingen, Germany;

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  • 正文语种 eng
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  • 关键词

    phosphatidylinositol synthase; phosphatidylinositol 4-kinase; phosphatidylinositol-4-phosphate 5-kinase; cooperativity; pectin secretion; pollen tubes;

    机译:磷脂酰肌醇合成酶;磷脂酰肌醇4-激酶;磷脂酰肌醇-4-磷酸5-激酶;协同作用;果胶分泌花粉管;

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