Prediction of the interacting surfaces in a trimolecular complex formed between the major dust mite allergen Der p 1, a mouse monoclonal anti-Der p 1 antibody, and its anti-idiotype

摘要

Background-Two mouse monoclonal antibodies (mAbs) have been described recently; namely, mAb 2C7 (IgG2bk), which is directed against the major house dust mite allergen Der p 1, and mAb 2G10 (IgG1k), which is an anti-idiotypic anti- body rised against mAb 2C7. The anti- idiotype mAb 2G10 does not block the binding of mAb 2C7 to Der p 1, which means that mAb 2C7 can simultaneously bind to Der p 1 and to mAb 2G10, thereby generating a trimolecular complex con- sisting of antigen-idiotype-anti-idiotype.