Determination of Total Selenium Content in Cereals and Bakery Products by Flow Injection Hydride Generation Graphite Furnace Atomic Absorption Spectrometry Applying in-situ Trapping on Iridium-Treated Graphite Platforms

摘要

A flow injection hydride generation graphite furnace atomic absorption spectrometric (FI-HG-GFAAS) method was applied to the determination of Se in Se-doped and undoped cereals and bakery products. For the purpose of doping, the soils used for the cultivation of the cereals were dosed with Se-doped foliar fertilizers. The samples were dissolved in a mixture of HNO3 and H2O2 solutions using microwave-assisted digestion. The decomposition of H2Se generated from the sample solutions and the trapping of elemental Se were performed at a temperature of 300 °C on an Ir-pretreated integrated graphite platform of a transversally heated graphite atomizer (THGA). For release of the trapped Se within a fairly short atomization time (5 s), an atomization temperature of 2200 °C was observed to be optimal. The overall efficiency of hydride generation, transport and trapping was ∼86%.