Application of PCR, rep-PCR and RAPD techniques for typing ofLactococcus lactis strains

摘要

A collection of 34 lactococcal strains were characterized using the polymerase chain reaction (PCR) for theacmA gene, and for the 16S rDNA gene, and DNA fingerprinting methods for randomly amplified polymorphic DNA (RAPD) and repetitive extragenic palindrome-PCR (rep-PCR). PCR experiments corroborated the genotypic identification ofLactococcus lactis strains by RAPD; rep-PCR did not distinguish betweenL. lactis subspecies. In some cases, phenotypic classification ofL. lactis subspecies did not correlate with genotypic characterization.