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首页> 外文期刊>Materials Research Bulletin >A convenient method of preparing gene vector for real time monitoring transfection process based on the quantum dots
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A convenient method of preparing gene vector for real time monitoring transfection process based on the quantum dots

机译：一种方便的制备基于量子点的基因载体用于实时监测转染过程的方法

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摘要

Nanoparticle carrier has been developed by combining water-soluble quantum dots and plasmid DNA expressed enhanced green fluorescent protein (EGFP) in a convenient and direct way. First the QDs with different surface charges were obtained by coating with amino and carboxyl terminals at different ratios. Then plasmid DNA was conjugated to QDs via electrostatic interaction. The resultant QDs-DNA complexes showed enhanced resistance to DNase I digestion. The following transfection experiments demonstrated that the transfection efficiency was dependent on the surface charges on QDs. The real ; time imaging of the transfection process showed that the nanoparticles experienced binding, penetrating the cell membrane and entering cytoplasm in the first 6 h of transfection. The green fluorescence of EGFP began to appear after 18 h transfection and plasmid DNA was fully expressed in the following 6 h. This new QDs-DNA platform showed great potential as new gene delivery carrier.
机译：通过将水溶性量子点和质粒DNA表​​达的增强型绿色荧光蛋白（EGFP）组合在一起，可以方便，直接地开发出纳米粒子载体。首先，通过以不同比例涂覆氨基和羧基末端获得具有不同表面电荷的量子点。然后，质粒DNA通过静电相互作用与QD偶联。所得的QDs-DNA复合物显示出对DNase I消化的增强抵抗力。以下转染实验表明，转染效率取决于QD上的表面电荷。真实;转染过程的时间成像显示，纳米颗粒在转染的前6小时内经历了结合，穿透细胞膜并进入细胞质的过程。转染18小时后，EGFP的绿色荧光开始出现，质粒DNA在随后的6小时内完全表达。这个新的QDs-DNA平台显示出作为新基因传递载体的巨大潜力。

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  • 来源
    《Materials Research Bulletin》 |2012年第11期|p.3330-3335|共6页
  • 作者

    Hai-Li Zhang; Ming-Zhen Zhang; Xiang-Yong Li; Min Wan; Yong-Qjang Li; Rong-Ying Zhang; Yuan-Di Zhao;

  • 作者单位

    Britton Chance Center for Biomedicai Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Department of Biomedcal Engineering, Wuhan 430074, PR China,Key Laboratory of Biomedicai Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Department of Biomedkal Engineering, Wuhan 430074, PR China;

    Britton Chance Center for Biomedicai Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Department of Biomedcal Engineering, Wuhan 430074, PR China,Key Laboratory of Biomedicai Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Department of Biomedkal Engineering, Wuhan 430074, PR China;

    Britton Chance Center for Biomedicai Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Department of Biomedcal Engineering, Wuhan 430074, PR China,Key Laboratory of Biomedicai Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Department of Biomedkal Engineering, Wuhan 430074, PR China;

    Key Laboratory of Biomedicai Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Department of Biomedkal Engineering, Wuhan 430074, PR China;

    Britton Chance Center for Biomedicai Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Department of Biomedcal Engineering, Wuhan 430074, PR China,Key Laboratory of Biomedicai Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Department of Biomedkal Engineering, Wuhan 430074, PR China;

    Key Laboratory of Biomedicai Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Department of Biomedkal Engineering, Wuhan 430074, PR China;

    Britton Chance Center for Biomedicai Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Department of Biomedcal Engineering, Wuhan 430074, PR China,Key Laboratory of Biomedicai Photonics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Department of Biomedkal Engineering, Wuhan 430074, PR China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    A. Nanostructures; B. Chemical synthesis; C. Photoelectron spectroscopy; D. Luminescence;

    机译：A.纳米结构;B.化学合成;C.光电子能谱;D.发光;

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