Encapsulation of enzymes in mesoporous host materials via the nonsurfactant-templated sol-gel process

摘要

A new, simple, one-step method for enzyme immobilization is demonstrated with encapsulating alkaline phosphates in mesoporous sol-gel silica. Since the pore size (e.g., ～30 A) of the mesoporous silica is much greater than that (e.g., ～15 A) in traditional microspourous sol-gel silica, enzymatic activity is increased by a factor of 2 to 10 because of easy diffusion of the substrate molecules. Compared to macroporous materials, the channel is narrow enough to prevent the enzyme from leaking out of its cage.