A hyaluronic acid/graphene oxide hydrogel for enhanced ex vivo expansion of cord blood-derived CD34(+) cells

摘要

HA/GO hydrogels were prepared by methacrylated GO (MeGO) and methacrylated HA (MeHA) via an in-situ free radical polymerization, and then a series of HA/GO hydrogels with highly porous structure were obtained. All hydrogels exhibited excellent water absorption ability. Additionally, the elastic moduli of hydrogels were clearly enhanced by GO. CD34(+) cells cultured on HA/GO-0.05 hydrogel showed higher expansion of CD34(+) cells and CD34(+) CD38(-) cells compared to the other three hydrogels. Therefore, HA/GO-0.05 hydrogels have a potential for clinical-applied ex vivo expansion of hematopoietic stem cells. (C) 2017 Elsevier B.V. All rights reserved.