Antihyperglycemic and anti-hyperlipidemic effect of biologically synthesized silver nanoparticles and G-sylvestre extract on streptozotocin induced diabetic rats-an in vivo approach

摘要

In recent times, nanomaterials being used in antidiabetic studies for their exclusive properties such as small size, more surface area, biocompatibility and enhanced solubility. In view of this, present study aimed to evaluate the antihyperglycemic potential of biologically synthesized silver nanoparticles (BSSNPs) and Gymnema sylvestre (GS) extract. The crystalline nature of the BSSNPs was confirmed by X-ray diffraction. The particle diameter size of BSSNPs determined by the Particle size analyzer. The SEM and HRTEM results revealed that the BSSNPs were spherical in shape. EDAX spectrum exhibit peaks for the presence of silver, carbon and oxygen atoms in the range of 1.0-3.1 keV. FT-IR reveals the binding properties of active bio-constituents responsible for capping and stabilizing BSSNPs. The results showed increased blood glucose, cholesterol, triglycerides, LDL, VLDL, huge loss in body weight, downturn in plasma insulin. The GS extract (200 mg/kg, 400 mg/kg), BSSNPs (100 mg/kg, 200 mg/kg) and Metformin 250 mg/kg were administered to the diabetic rats. BSSNPs at dose level of 200 mg/kg (b. wt p.o.) showed significant inhibition of (p < 0.001) blood glucose levels and lipid profile as compared with GS extract treated group. These findings suggested that BSSNPs having potent anti-hyperglycemic and anti-hyperlipidemic activity as compared to the crude extract.(C) 2017 Elsevier B.V. All rights reserved.