Phytofabrication and encapsulated of silver nanoparticles from Gloriosa superba

摘要

The present study has been investigated for the formation of silver nanoparticles (AgNPs) toxicity was reduced by a biological route employing methanolic extract of Gloriosa superba seed (MGsS) extract. AgNPs protected with -CH2- group, -NH2- groups and -CO- groups of colchicines and colchicine derivatives of Gloriosa superba seed extract are not cytotoxic, while, by contrast bare AgNPs have been found to be rather toxic. Capping and efficient stabilization of silver ions by MGsS confirmed by FTIR analysis. TEM analysis also revealed that the edges of the particles were lighter than the centers, confirmed that the bioorganic compounds such as alkaloids (colchicines) in MGsS capped the silver NPs contributing to reduction of Ag+ ions to Ag-0. Formation of AgNPs was confirmed by UV-visible absorbance peaks at 360 and 440 nm clearly indicated the interaction between AgNPs and biomolecules present in the MGsS. The XRD study confirmed the crystalline face-centered cubic lattice of AgNPs of methanolic extract of Gloriosa superba seed (AgMGsS). The average size of the nanoparticles was determined to be about 35-42 nm.