phylogenetic diversity of marine coastal picoplankton 16S rRNA genes cloned from the continental shelf off Cape Hatteras, North Carolina

摘要

The phylogenetic diversity of a continental-shelf picoplankton community was examined by analyzing 16S ribosomal RNA (rRNA) genes amplified from environmental DNA with bacterial-specific primers and the polymerase chain Reaction (PCR). Picoplankton populations collected from the pycnocline (10 m) over the eastern continental shelf of The United States near Cape hatters, North Carolina, served as the source of bulk nucleic acids used in this study. A large proportion of the 169 rDNA clones recovered (33/100) were related to plastid 16S rRNA genes, including Plastids from both chromophyte and chlorophyte algae.