Osteogenic Effect of Genistein on In Vitro Bone Formation by Human Bone Marrow Cell Culture - For Development of Advanced Bio-Artificial Bone -

摘要

The effect of genistein, a soybean isoflavone, on new bone formation by bone marrow cells from mature humans was examined. After informed consent was obtained from a 55-year-old woman with osteoporosis and lumbar spondylosis deformans, bone marrow cells were collected from her ilium, cultured in the standard medium of MEM containing fetal calf serum and then cultured with or without the addition of genistein to the bone-forming medium containing dexamethasone etc.. In humans, when genistein was added to the bone-forming medium, genistein (10~(-7) M and 10~(-8) M) caused a significant increase in the levels of alkaline phosphatase avtivity and DNA content compared with cells not cultured in genistein. In conclusion, genistein was found to promote bone formation at lower concentrations, and thus may be useful as a bone formation-promoting factor.