DNA Single Strands Tethered to Fused Quartz/Water Interfaces Studied by Second Harmonic Generation

摘要

DNA forms the basis of all life processes and has received significant attention in many areas of science.While intense research has focused on characterizing DNA at interfaces for the purpose of biodiagnostics,many of these studies require the synthesis of oligonucleotides labeled with fluorescent,electrochemical,radioactive,or nanoparticle tags to afford detection.Label-free techniques for DNA detection,such as surface plasmon resonance spectroscopy,atomic force microscopy,X-ray spectroscopies,and Fourier transform infrared (FTIR) spectroscopy,eliminate the synthetic steps necessary for labeling oligonucleotides but often require surfaces with high dielectric constants.