Composite Alignment Media for the Measurement of Independent Sets of NMR Residual Dipolar Couplings

摘要

Residual dipolar couplings(RDCs)have become one of the standard tools employed for biomolecular NMR structure determination due to their sensitivity to minute changes in the relative orientation of even spatially remote bonds.However,as a single measured RDC does not correspond to a unique bond orientation,RDC-based restraints are most easily introduced after an initial structural model has been derived using other NMR data.This ambiguity in the interpretation of the RDCs can be lifted using one of several more data intensive approaches.A particularly fruitful route for overcoming orientational ambiguity is to measure RDCs utilizing several different aligning media.