Artificial Polypeptide Scaffold for Protein Immobilization

摘要

Protein microarray technologies are beginning to advance the field of proteomics by providing miniaturized platforms to probe the interactions and functions of proteins.Presenting proteins in dense arrays enables the rapid screening of thousands of molecular events in a single experiment.This capability should facilitate the elucidation of protein profiles in organisms,the discovery of novel protein functions,and the development of systems-level understanding of biological phenomena.The utility of microarrays to probe protein-protein interactions has been demonstrated by Zhu et al.,where new calmodulin- and phospholipid-binding proteins were identified by screening a full scale yeast proteome microarray.More recently,Nielsen et al.used antibody microarrays to profile the activation of receptor tyrosine kinases and analyze signal transduction networks in mammalian cells.