DNA-Catalyzed Transfer of a Reporter Group

摘要

DNA-templated reactions are emerging as a general approach to control the reactivity of synthetic molecules by modulating the effective molarity.DNA templates align the reactive groups of these molecules to allow fast and selective reactions at reactant concentrations that are much lower than those required for conventional synthesis.Among various types of chemistries,most DNA-directed reactions involve the covalent ligation of two modified oligonucleotide strands.In general,ligation reactions suffer from an increased affinity of the product to the DNA template.This causes product inhibition and prevents high catalytic activity of the nucleic-acid template.However,catalysis would be a valuable asset,particularly in DNA and RNA detection chemistries.