Simultaneous Determination of Protein Backbone Structure and Dynamics from Residual Dipolar Couplings

摘要

Standard approaches to protein structure determination propose high-resolution models of a single configuration and take little or no account of conformational fluctuation.It is,however,accepted that accurate knowledge of the dynamic behavior of proteins is equally crucial to understanding their function.1 While motional,properties are often measured independently of conformation,simultaneous determination of both aspects would provide essential details of the conformational space sampled by the native protein as well as more accurate average conformations.