Lanthanide-based Protease Activity Sensors For Time-resolved Fluorescencernmeasurements

摘要

Time-resolved fluorescence measurements with long-lifetime luminescent lanthanide complexes have attracted great attention from scientists. This is because the time-resolved luminescence signals are highly sensitive and are scarcely affected by other fluorescent compounds that coexist in the sample, which often interfere with steady-state fluorescence measurements. This method is quite useful, especially in high-throughput drug screening. Protease inhibitors can be important drug targets for diverse diseases, including cancer, AIDS, inflammatory disorders, and so on. Therefore, lanthanide-based luminescent sensors that detect protease activities assume great significance.