New Aldehyde Tag Sequences Identified by Screening Formylglycine Generating Enzymes in Vitro and in Vivo

摘要

Formylglycine generating enzyme (FGE) was identified in 2003 as the posttranslational machinery that activates type I sulfatases in eukaryotes. The enzyme oxidizes a cysteine residue within a ～13 amino acid consensus sequence, also termed the "sulfatase motif, forming an aldehyde-bearing formylglycine (FGly) residue (Figure 1) that is critical for the sulfatases' catalytic function. In eukaryotes, FGE requires a minimal submotif, CxPxR, that is highly conserved among all type I sulfatases.