Selective Detection of Protein Secondary Structural Changes in Solution Protein-Polysaccharide Complexes Using Vibrational Circular Dichroism (VCD)

摘要

The challenge of storing and transmitting the vast quantities of information required for intercellular communication in living organisms was met by employing complex carbohydrates. The structural differences of the constituent sugars and manifold modes of connecting them to form oligo- and polysaccharides resulted in structural diversity with vast potential information content. Con-formational differences of carbohydrates are brought to bear through interactions with proteins, often accompanied by protein confor-mational changes following binding, resulting in altered activity.