Double-Nucleus Enhanced Recoupling for Efficient ~(13)C MAS NMR Correlation Spectroscopy of Perdeuterated Proteins

摘要

Recent progress in solid-state magic-angle-spinning (MAS) NMR methodology has significantly contributed to the prospects for obtaining structural insight into "difficult", biologically relevant materials such as membrane proteins and protein fibrils. In the quest for higher resolution, the use of perdeuterated proteins has been advocated, yielding spectra with liquid-state-like resolution in both the ~1H and ~(15)N/~(13)C dimensions.