MgF_3~- and α-Galactose 1-Phosphate in the Active Site of β-Phosphoglucomutase Form a Transition State Analogue of Phosphoryl Transfer

摘要

Enzyme catalyzed phosphoryl transfer reactions play integral roles in metabolism, regulation, and cell signaling and are associated with the largest enzymatic rate enhancements yet known. Tri-fluoromagnesate (MgF_3~-) has recently been identified as a trigonal planar mimic for the PO_3~- group in transition states (TS) for several of these enzymes. Its occurrence is likely to be even more widespread since transition state analogue (TSA) structures previously thought to contain AlF_3 most likely instead contain MgF_3~-.