An Electrochemical Sensor for the Detection of Protein-Small Molecule Interactions Directly in Serum and Other Complex Matrices

摘要

Here we have demonstrated an apparently general method for the electrochemical detection of macromolecules that interact strongly with specific small molecule targets. Using the small molecule recognition elements biotin and digoxigenin, we demonstrate detection of subnanomolar to low nanomolar concentrations of streptavidin and anti-digoxigenin antibodies directly in blood serum and other complex sample matrices.