A Mitochondrial-Targeted Two-Photon Probe for Zinc Ion

摘要

We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions ([Zn~(2+)]_m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn~(2+) with a dissociation constant (-K_d~TP) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of [Zn~(2+)]_m in a rat hippocampal slice at a depth of 100—200μm without interference from other metal ions through the use of two-photon microscopy.