DESⅡ REACTION MECHANISM PROBED WITH FLUORINATED ANALOGUES

摘要

DesⅡ catalyzes a key step in the synthesis of macrolide antibiotics. The enzyme consumes S-adenosyl-L-methionine (SAM) in a deamination reaction required for the formation of TDP-D-desosamine, an unusual sugar that is required to glycosylate the antibiotics. But how this enzyme functions is unclear.