Biosensor Sensitive to Hydrogen Peroxide via Methylene Blue Incorporated into Nafion Film as an Electron Transfer Mediator

摘要

Biosensor sensitive to hydrogen peroxide was made via methylene blue in Nafion gel as an electron transfer mediator. Cyclic voltomeetry and chronoamperometry were for the first time used to illustrate the suitability of electrical communication between immobilized horseradish peroxidase and a glassy carbon elec- trode via methylene blue incorporated into a (Nafion) perfluorosulfonic acid cation-exchange polymer film. The methylene blue mediator was strongly reatined inside Nafion film through hydrophobic interactions.