Effect of Cerium on Expression and Activity of MMP-9 from Human Carcinoma of Bladder Cell Line

摘要

The effects of Ce~(4+) on cell survival, expression and activity of matrix metalloproteinase-9 (MMP-9) with MTT colorimetry and gelatin zymography assays in human bladder carcinoma cell line was studied. The results indicate that the lower concentration of Ce~(4+) (0.01 mmol centre dot L~(-1)) has no influence on cell growth, but inhibits extremely expression of MMP-9 and increases its activity, whereas the higher concentration of Ce~(4+) (1.0 mmol centre dot L~(-1)) can inhibit all of them. The results raise the possibility that Ce~(4+) may have beneficial effects in attenuating invasion and metastasis of malignant tumors.