An Improved Method for the Synthesis of Nucleoside Triphosphate Analogues

摘要

Nucleoside monophosphates,when activated by trifluoro-acetic anhydride and N-methylimidazole,efficiently couple with a variety of electron-deficient diphosphonates in a reproducible and efficient manner(<2 h,>72% isolated yield).Unlike traditional methods for the preparation of nucleoside 5'-beta,gamma-methylenetriphosphate analogues,there is no requirement for predrying,or conversion to specific salt forms,of commercially available nucleoside monophosphate starting materials.